3-Indoxyl Phosphate: an Alkaline Phosphatase Substrate for Enzyme Immunoassays with Voltammetric Detection

1998 ◽  
Vol 10 (4) ◽  
pp. 249-255 ◽  
Author(s):  
César Fernández-Sánchez ◽  
Agusťín Costa-Garcı´a
Keyword(s):  
Alkaline Phosphatase ◽  
Enzyme Immunoassays ◽  
Voltammetric Detection ◽  
Alkaline Phosphatase Substrate ◽  
Phosphatase Substrate

Detection of salmonellas by DNA hybridization with a fluorescent alkaline phosphatase substrate

1992 ◽  
Vol 72 (5) ◽  
pp. 393-399 ◽  
Author(s):  
R. J. Cano ◽  
M.J. Torres ◽  
R.E. Klem ◽  
J.C. Palomares ◽  
J. Casadesus
Keyword(s):  
Alkaline Phosphatase ◽  
Dna Hybridization ◽  
Alkaline Phosphatase Substrate ◽  
Phosphatase Substrate

scholarly journals Novel Live Alkaline Phosphatase Substrate for Identification of Pluripotent Stem Cells

2012 ◽  
Vol 8 (3) ◽  
pp. 1021-1029 ◽  
Author(s):  
Upinder Singh ◽  
Rene H. Quintanilla ◽  
Scott Grecian ◽  
Kyle R. Gee ◽  
Mahendra S. Rao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Alkaline Phosphatase ◽  
Pluripotent Stem Cells ◽  
Alkaline Phosphatase Substrate ◽  
Phosphatase Substrate

Utilizing ultrafiltration to remove alkaline phosphatase from clinical analyzer water

2006 ◽  
Vol 44 (5) ◽  
Author(s):  
Julien Bôle ◽  
Stéphane Mabic
Keyword(s):  
Alkaline Phosphatase ◽  
Water Samples ◽  
Bacterial Contamination ◽  
Pure Water ◽  
Experimental Conditions ◽  
Enzyme Immunoassays ◽  
Selection Of

AbstractAlkaline phosphatase (ALP) conjugated to antibodies is often used in enzyme immunoassays (EIAs). These assays are notably sensitive to experimental conditions. A possible source of interference is bacterial ALP, which is released when bacterial contamination occurs in clinical analyzers. Preliminary experiments led to the selection of a detection kit, ALP source, and specific types of tubes for collecting water samples and performing assays. The release of ALP from various strains of bacteria identified in pure water was demonstrated (10–30×10

scholarly journals Use of a new fluorogenic phosphatase substrate in immunohistochemical applications.

1995 ◽  
Vol 43 (1) ◽  
pp. 77-83 ◽  
Author(s):  
K D Larison ◽  
R BreMiller ◽  
K S Wells ◽  
I Clements ◽  
R P Haugland
Keyword(s):  
Alkaline Phosphatase ◽  
Stokes Shift ◽  
High Magnification ◽  
Adult Zebrafish ◽  
Large Stokes Shift ◽  
Retinal Tissue ◽  
Bright Yellow ◽  
Immunohistochemical Techniques ◽  
Phosphatase Substrate

We used the phosphatase substrate 2-(5'-chloro-2'-phosphoryloxyphenyl)-6- chloro-4-[3H]-quinazolinone, with standard alkaline phosphatase-mediated immunohistochemical techniques, to visualize a number of antibodies that bind to adult zebrafish retinal tissue. This compound, known as the ELF (enzyme-labeled-fluorescence) phosphatase substrate, produces a precipitate that fluoresces at approximately 500-580 nm (bright yellow-green). We show that the precipitated product from the ELF phosphatase substrate has a number of characteristics that make it superior to fluorescein-labeled secondary reagents. The staining produced with the ELF substrate is much more photostable than that produced by fluorescein-labeled secondary reagents, thus allowing time to examine, focus, and photograph the ELF-labeled tissue under high magnification. Moreover, the ELF precipitate exhibits a Stokes shift of greater than 100 nm, a characteristic that has enabled us to overcome the problem of distinguishing signal from background in this autofluorescent tissue. In addition, we show that the ELF product's large Stokes shift makes the ELF substrate ideal for multicolor applications.

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